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| Facincani,Agda Paula; Ferro,Jesus Aparecido; Pizauro Jr.,João Martins; Pereira Jr.,Haroldo Alves; Lemos,Eliana Gertrudes de Macedo; Prado,Alessandro Luis do; Ferro,Maria Inês T.. |
| The objective of this work was to assess the functionality of the glycolytic pathways in the bacterium Xylella fastidiosa. To this effect, the enzymes phosphoglucose isomerase, aldolase, glyceraldehyde-3-phosphate dehydrogenase and pyruvate kinase of the glycolytic pathway, and glucose 6-phosphate dehydrogenase of the Entner-Doudoroff pathway were studied, followed by cloning and expression studies of the enolase gene and determination of its activity. These studies showed that X. fastidiosa does not use the glycolytic pathway to metabolize carbohydrates, which explains the increased duplication time of this phytopatogen. Recombinant enolase was expressed as inclusion bodies and solubilized with urea (most efficient extractor), Triton X-100, and TCA.... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Enolase; Entner-Doudoroff; Enzyme; Glycolysis; Xylella fastidiosa. |
| Ano: 2003 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000200015 |
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| Silva,Josiane Aparecida Gonçalina da; Paula,Daphine Ariadne Jesus de; Silveira,Marcelo Marques da; Silva,Maria Cristina da; Brandão,Laila Natasha Santos; Pitchenin,Leticia Camara; Nakazato,Luciano; Dutra,Valéria. |
| Conidiobolus lamprauges é um fungo zigomiceto patógeno de humanos e animais, causador da conidiobolomicose, caracterizada por uma rinossinusite crônica granulomatosa severa. A capacidade de se adaptar e crescer a altas temperaturas são sugeridos como um atributo de virulência em fungos que infectam animais e humanos, no entanto, em C. lamprauges, pouca informação é disponível sobre esse aspecto. O objetivo deste trabalho foi identificar genes com expressão diferencial em C. lamprauges cultivado a 30° e 37°C através da técnica de Análise de Diferença Representacional (RDA). Após análise e sequenciamento de um conjunto de 120cDNAs, identificou-se uma enzima glicolítica denominada enolase, diferencialmente expressa a 37°C. Esse gene exerce funções... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Expressão diferencial; Enolase; C. lamprauges. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782012000900015 |
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| Marchal,S.; Torrent,J.; Masson,P.; Kornblatt,J.M.; Tortora,P.; Fusi,P.; Lange,R.; Balny,C.. |
| The pressure behavior of proteins may be summarized as a the pressure-induced disordering of their structures. This thermodynamic parameter has effects on proteins that are similar but not identical to those induced by temperature, the other thermodynamic parameter. Of particular importance are the intermolecular interactions that follow partial protein unfolding and that give rise to the formation of fibrils. Because some proteins do not form fibrils under pressure, these observations can be related to the shape of the stability diagram. Weak interactions which are differently affected by hydrostatic pressure or temperature play a determinant role in protein stability. Pressure acts on the 2º, 3º and 4º structures of proteins which are maintained by... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: High pressure; Prion protein; Amyloid; Ataxin; Enolase; Butyrylcholinesterase. |
| Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000800004 |
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